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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may possibly add to tumorigenesis. This protein was discovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital position in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two additional splice variants have been noted but comprehensive nucleotide sequence data just isn't however available.
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Blocking peptides are peptides that bind precisely into the goal antibody and block antibody binding. These peptide normally incorporates the epitope regarded through the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guideline outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide no more bind into the epitope to the focus on protein. By comparing the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is particular.
Target Info Cyclin E belongs to the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every more info mitotic function. Cyclin E types a posh with and functions as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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